Advanced glycation end products promote the metastasis of colorectal cancer cells via centrosome amplification mediated by KLF5-CEP57L1 axis

Emerging evidence suggests that diabetes and centrosome amplification (CA) are associated with metastasis and in vitro invasion of cancer cells, respectively. We have reported that diabetes promotes CA; thus, we sought to determine whether CA may serve as a biological link between diabetes and metastasis. Our study demonstrated that advanced glycation end products (AGEs) in vitro simultaneously promoted CA, migration and invasion of HCT116 colorectal cancer cells, with higher levels of CA in migrated cells. Herein, we provided the original western blot and transcriptomic profling results.

涌现的证据表明，糖尿病与中心体扩增（CA）分别与癌症的转移及体外癌细胞侵袭相关联。本研究已报道糖尿病可促进CA；因此，我们旨在探究CA是否可能成为糖尿病与转移之间的生物学桥梁。我们的研究结果表明，体外的高级糖基化终产物（AGEs）同时促进了HCT116结直肠癌细胞中CA、迁移和侵袭，且迁移细胞中CA水平较高。在此，我们提供了原始的蛋白印迹和转录组分析结果。