Impact of postures on Cobb angle measurements in scoliosis mouse models

This data set concludes all the images, charts, statistics and reference that occurred in my thesis. Here is the abstract.Scoliosis is defined by a Cobb angle measurement that exceeds 10° from the anterior-posterior standing radiograph. Its diagnosis and management decisions rely on the stringent assessment of the curve magnitude.There are quadrupedal animal models like pigs, rabbits and rats, bipedal animal models like chickens and non-human primates. There are also guppy and zebrafish which are widely used in genetic model establishment. Mouse is suitable for either - the mechanical model or the genetic model, and the genetic background is clear with a relatively high gene homology, which makes it a promising animal model for future scoliosis study.In humans, the effect of positioning error on Cobb angle measurements and the measurement error expected from variations in imaging have been investigated intensively. As there is no study that describes these two kinds of error in animal models, the study objective is to test the effect of postures on the angle measurement as well as to establish a standard for mouse imaging.In present study, we first collected 77 mice to do radiography. During the experiment, we described the criteria for a standard right posture in terms of the symmetry of body. We found out that the incidence of scoliosis in mouse population was around 28.6%. There was a big variation in curve magnitude as well. We took weekly X-rays for 24 mice and found out that the variation of multiple-time measurement was also very big. In the second part of the study, we collected 82 mice and designed 14 postures that may vary during imaging. The results showed that tilting of the head (>30°) and the imbalance of the pelvis would greatly affect the curve magnitude with effect size (Glass’s delta) over 1.50 which is huge according to the definition.Our work exemplified the importance of standard protocol during imaging when using animal model in the scoliosis study. This is important for all imaging studies in these animals. We recommend utilizing this standard in studying various disorders of the spine.

本数据集汇总了论文中出现的所有图像、图表、统计数据及参考资料。以下是摘要。脊柱侧弯是以前后站立位X光片测得的Cobb角度超过10°为定义的。其诊断和管理决策依赖于对弯曲程度的严格评估。存在四足动物模型，如猪、兔和鼠，两足动物模型，如鸡和灵长类动物。此外，还有斗鱼和斑马鱼，它们在遗传模型建立中得到了广泛应用。小鼠既适用于机械模型，也适用于遗传模型，其遗传背景清晰，基因同源性相对较高，这使得它成为未来脊柱侧弯研究的有希望的动物模型。在人类中，对姿势误差对Cobb角度测量的影响以及成像变化预期带来的测量误差进行了深入研究。由于目前尚无研究描述动物模型中的这两种误差，本研究旨在测试姿势对角度测量的影响，并建立小鼠成像的标准。在本研究中，我们首先收集了77只小鼠进行X光摄影。在实验过程中，我们以身体的对称性为标准，描述了标准右侧位的判定标准。我们发现小鼠种群中脊柱侧弯的发病率约为28.6%。弯曲程度存在很大差异。我们对24只小鼠进行了每周的X光检查，并发现多次测量的变化也非常大。在研究的第二部分，我们收集了82只小鼠，并设计了14种可能在成像过程中发生变化的姿势。结果表明，头部倾斜（>30°）和骨盆不平衡会极大地影响弯曲程度，其效应量（Glass的delta）超过1.50，这在定义上是一个巨大的数值。我们的研究展示了在脊柱侧弯研究中使用动物模型时，标准化操作规程的重要性。这对于这些动物的成像研究具有重要意义。我们建议在研究脊柱的各类疾病时，采用这一标准。