Adam, Kolyfetis et al., 2021. Supplementary Data.

Compressed Supplementary Data files for (Adam, Kolyfetis, et al., 2022). File descriptions are given below and in more detail in the readme file. This is version 20220411. <br> Supplementary Data 1 Data regarding all DUF phylogenies (taxonomic distributions, HMM profiles, homology search outfiles with and without e-value threshold, alignments, trimmed alignments, phylogeny runs), plus local genome database records. <br> Supplementary Data 2 Data regarding methanogenesis markers phylogenies from (Borrel et al., 2019) (HMM profiles, DIAMOND query sequences, homology search outfiles with and without e-value threshold, alignments, trimmed alignments, and phylogeny runs). <br> Supplementary Data 3 Data regarding methanogenesis marker phylogenies from (Gao &amp; Gupta, 2007) and phylogenies of Wood-Ljungdahl pathway and methyltransferase proteins (HMM profiles, DIAMOND query sequences, homology search outfiles with and without e-value threshold, alignments, trimmed alignments, and phylogeny runs). <br> Supplementary Data 4 Data regarding congruence tests and the concatenation phylogeny of Mcr. <br> Supplementary Data 5 Data regarding congruence tests and the concatenation phylogeny of Mtr. <br> Supplementary Data 6 Data regarding phylogenies of additional Eha, Ehb and Hcg proteins (HMM profiles, DIAMOND query sequences, homology search outfiles with and without e-value threshold, alignments, trimmed alignments, and phylogeny runs). <br> Supplementary Data 7 Data regarding congruence tests and the concatenation phylogeny of Eha. <br> Supplementary Data 8 Data regarding congruence tests and the concatenation phylogenies of Ehb. <br> Supplementary Data 9 Data regarding all rooting analyses on the concatenations and the methyltransferases genes. <br> Supplementary Data 10 Data regarding gene and site concordance factor calculations for all concatenations. <br> Supplementary Data 11 Data regarding all Ancestral Sequence Reconstruction runs, homology searches of the ancestral sequences, trimmed ancestral sequences, and homology modeling of McrA. <br> Supplementary Data 12 Data regarding congruence tests and the concatenation phylogenies of the Hcg proteins. <br> Supplementary Data 13 Data regarding site-specific rate calculations, benchmarks, and all related statistical tests. Transmembrane segment prediction runs and statistical tests for their relationship eith site-specific rates. <br> Supplementary Data 14 Data regarding the phylogenetic runs for the taxonomic placement of NRA7 (Archaeoglobi) and Bathyarchaeia metagenomic bins. Also data regarding AAI/ANI, biogeography, and 16S analyses, plus CheckM outfiles. <br> Supplementary Data 15 Data regarding the results of the metabolic prediction (BlastKOALA/dbCAN2/HydDB/MEROPS) for the genomes studied. <br> Changelog 1) Numbering of Supplementary Data files changed to corrrespond to the revised manuscript. 2) Added MtsB phylogenetic analyses (Supplementary Data 3) and rooting (Supplementary Data 9). 3) Added taxonomic analyses (Supplementary Data 14) and metabolic predictions (Supplementary Data 15) for Hydrothermarchaeota and Geothermarchaeales. 4) Added phylogenetic analyses combining the large catalytic subunits of [NiFe] hydrogenase groups 4h (Eha) and 4i (Ehb) (Supplementary Data 6). 5) Added Pearson and updated Spearman correlations for rate calculation benchmarks (Supplementary Data 13). Added Spearman correlations for desaturation dataset benchmarks (Supplementary Data 14).