Evolvability

<h1>Introduction</h1>
This record contains data accompanying the paper:
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Experimental evolution of evolvability
Michael Barnett, Lena Zeller, Paul B. Rainey
<a href=https://doi.org/10.1101/2024.05.01.592015>doi:10.1101/2024.05.01.592015</a>
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<h1>Content of expevolv.zip</h1>
The folder 'Population B whole-genome seq' contains all fastQ files derived from whole-genome re-sequencing of clones within 'meta-population B' that were used to identify and order mutations. A GenBank file 'OV986001-SBW25_genome' provides the appropriate reference genome for mapping these sequences.
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Names of fastQs files accord to the table 'interactive-pop-B.csv', which itself is the input used to visualise lineage dynamics of meta-population B within the paper via the software 'Colgen'. The interactive lineage visualization of meta-population B can be accessed at <a href=https://micropop.evolbio.mpg.de/data/evolve>https://micropop.evolbio.mpg.de/data/evolve</a>.
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Colgen can be found here:
<a href=https://gitlab.com/ecoevomath/colgen>https://gitlab.com/ecoevomath/colgen</a>
And its accompanying paper here:
<a href= https://doi.org/10.1101/2024.05.10.593459>doi:10.1101/2024.05.10.593459</a>
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The folder 'Sanger seq from T10-M3 cel+ transposon mutagenesis' contains all Sanger sequences used to identify transposon insertion sites following transposon mutagenesis. These can be mapped to the GenBank reference file.

<h1>Contents of genetic_constructs.zip</h1>
The folder 'Genetic constructs' contains annotated GenBank files for the novel donor plasmids used in this study to introduce genetic changes.