Bulk RNA-sequencing of mouse prostate tumors initiated by transplantation of engineered organoids.

Purpose: Profile global expression of prostatic tumors to compare with published datasets. Methods: Prostate tumors were flash frozen followed by processed for total RNA using the RNeasy kit (Qiagen). Samples were submitted for polyA library preparation and bulk RNA-sequencing. Conclusions: Global gene expression profiling and comparison of prostate tumor genotypes suggests differential enrichment of neuroendocrine signatures that match established human neuroendocrine prostate cancer expression signatures. To investigate the molecular landscape of transplanted PtPM and RPM orthotopic prostate tumors, n = 10 PtPM and n = 8 RPM tumors isolated at early or late time points were subjected bulk RNA-sequencing.