Transcriptome analysis of systemic defense priming in Arabidopsis thaliana locally inoculated with Pseudomonas syringae pv tomato DC3000 AvrRpm1

Defense activation at local pathogen challenged leaves leads to systemic acquired resistance (SAR) with defense-related genes primed for a subsequent stimulus at distal unchallenged (systemic) leaves. Our previous studies implied the possible involvements of transcription-permissive histone modification H3K4me3 and transcription-repressive H3K27me3 in systemic immunity. Our studies found that ATX1 (responsible for H3K4me3 deposition) and CLF-PRC2 (responsible for H3K27me3 deposition) both contribute to systemic defense priming and SAR. To obtain an overview for CLF-PRC2-dependent systemic defense priming, we conducted RNA-seq analysis in systemic leaves of Arabidopsis thaliana WT Col-0 and clf-28 plants. Plants were locally inoculated with Pseudomonas syringae pv tomato DC3000 AvrRpm1 or mock. Two days after the bacterial inoculation, non-challenged systemic leaves were harvested at 0, 0.5 and 3 hours after the second treatment with distilled water and subjected to RNA-seq analysis.