Selection and validation of reference genes for measuring gene expression in Toona ciliata under different experimental conditions by quantitative real-time PCR analysis
收藏国家林业和草原科学数据中心2022-12-19 更新2024-03-06 收录
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The experimental results showed that TUB-α was the most stably expressed reference gene across all
samples and UBC17 was the most stable in leaves and young stems under Hypsipyla robusta (H. robusta) and methyl
jasmonate (MeJA) treatments. In addition, PP2C59 and UBC5B were the best-performing genes in leaves under H.
robusta treatment, while HIS1 and ACT7 were the best reference genes in young stems. The two best reference
genes were 60S-18 and TUB-α after treatment at 4 °C. The expression of HIS6 and MUB1 was the most stable under
PEG6000 treatment. The accuracy of the selected reference genes was verified using the transcription factor MYB3
(TcMYB3) gene.
提供机构：
国家林业和草原科学数据中心
创建时间：
2022-12-19
