RNA-seq on primary human kidney epithelial cells with oleate treatment and PLIN2 knockdown

Here we investigated the transcriptomic changes induced by oleic acid treatment on human Primary Renal Proximal Tubule Epithelial Cells (RPTECs) with RNA-seq. We also profiled cells with oleic acid treatment after PLIN2 gene knockdown with siRNA treatment. Overall design: There are a total of 15 samples derived from 5 groups with 3 replicates per group. Sample 1-3: RPTECs treated with non-targeting control siRNA (NTsiRNA). Sample 4-6: RPTECs treated with NTsiRNA and processed for 6-hour oleate-BSA stimulus. Sample 7-9: RPTECs treated with PLIN2 siRNA and processed for 6-hour oleate-BSA stimulus. Sample 10-12: RPTECs treated with NTsiRNA and harvested at 2 days after 6-hour oleate-BSA stimulus. Sample 13-15: RPTECs treated with PLIN2 siRNA and harvested at 2 days after 6-hour oleate-BSA stimulus. Libraries were generated with the poly-A selection method and sequenced with the NovaSeq 6000 S4 platform (2×150 bp) at a target of 30 million reads per library.