Inter-kingdom gut microbiome and resistome of the cockroach Blattella germanica

In this experiment, we study the effect of the antibiotic kanamycin on the inter-kingdon hindgut microbiome in Blattella germanica, specifically its transmission, assembly and functions, was studied using a metagenomic analysis. The adult females were treated with kanamycin during the adult phase in the first generation. Individuals derived from these in the second generation for each treatment with antibiotics were 1) treated with the same antibiotic but starting in the nymphal period, 2) not treated with the antibiotic, or 3) not treated with the antibiotic but supplied with feces from a population control. Samples of nymphs and adult females were taken through the two generations for experimental and control condicions. After the dissection of insects, the DNA was extracted from the hindgut and the metagenomics analyses was performed to study the structure and function of its gut microbiome along development as well as the characterization of its resistome.