Improved retinal organoid differentiation by modulating signaling pathways revealed by comparative transcriptome analyses with development in vivo

This study provides analysis of developing mouse retina organoids derived from induced pluripotent stem cells . RNA-seq profiling was performed on 2 time points during retinal organoids supplemented with docosahexaenoic acid (DHA), linoleic acid (LA), and/or FGF1. Alignment, transcript and gene quantitation, and normalization was performed. Overall design: Retina organoids were collected in duplicate or triplicate at 2 time points during differentiation (day (D) 26 and 32) with and without DHA and FGF1 supplementation. Please note that the Gene_Normalized-CPM.tsv contains 18 data columns as 5 samples were eliminated for the particular analysis. The other processed data files contain the complete data for all 23 samples.