Parameters for cAMP binding to CRP.

The data in Fig 3 can be characterized using a single set of dissociation constants for the WT, D, and S subunits whose values and standard errors are shown. To excise parameter degeneracy, the active-inactive free energy difference ϵ and the cAMP interaction energy in the active state are absorbed into the active state dissociation constants in the MWC model (Eqs (2) and (3)). Similarly, ϵ is absorbed into the KNF dissociation constants (Eqs (6) and (7)).