HT-SELEX and genomic HT-SELEX rawdata for experiments performed with 4 C2H2 zinc finger transcription factors for the study: " Alternate binding modes of human C2H2 TFs". Alternate binding modes of human C2H2 TFs

Study describes (Abhi and al., please add something here about the paper). High throughput Systematic Evolution of Ligands Through Exponential Enrichment (HT-SELEX) method was modified from approaches described in Jolma et al.(2010, 2013 and 2015) and Nitta et al. 2015. (PMID: 20378718, PMID: 23332764, PMID: 26550823 and PMID: 25779349). In this study we also performed new type of assays called Genomic HT-SELEX (GHT-SELEX), that uses enzymatically fragmented human genomic DNA instead of random DNA sequence. Method has similarities with both Affinity-seq method by Walker et al. 2015 (PMID: 26351520) and earlier genomic SELEX methods such as methods by Singer et al. 1997 and Zimmermann et al. 2010 (PMID: 9016629 and PMID: 20541015m, respectively). Information about the experiments is encoded both in metadata and in the originally submitted sample file names. In the sample names the information is separate by underscore ""_"" symbols. For example sample named "ZNF121_TT40NTACAGC_Lysate_BatchAATA_Cycle3_R1.fastq.gz"" Means that it is derived for experiment using: 1) Protein ZNF121, 2) selection ligand where the randomised region is flanked by TT and TACAGC residues , 3) It is from experimental batch ""AATA"", and 4) From the first selection cycle of the assay. GHT-SELEX samples do not contain flanking barcodes. They were instead multiplexed with Illumina's i7 and i5 barcode system during final library preparation and have been derived from same fragmented pool.