Data from: Survival, gene and metabolite responses of Litoria verreauxii alpina frogs to fungal disease chytridiomycosis

PLEASE NOTE, THESE DATA ARE ALSO REFERRED TO IN ANOTHER PUBLICATION. PLEASE SEE http://dx.doi.org/10.1111/mec.14493. The fungal skin disease chytridiomycosis has caused the devastating decline and extinction of hundreds of amphibian species globally, yet the potential for evolving resistance, and the underlying pathophysiological mechanisms remain poorly understood. We exposed 406 naïve, captive-raised alpine tree frogs (Litoria verreauxii alpina) from multiple populations (one evolutionarily naïve to chytridiomycosis) to the aetiological agent Batrachochytrium dendrobatidis in two concurrent and controlled infection experiments. We investigated (A) survival outcomes and clinical pathogen burdens between populations and clutches, and (B) individual host tissue responses to chytridiomycosis. Here we present multiple interrelated datasets associated with these exposure experiments, including animal signalment, survival and pathogen burden of 355 animals from Experiment A, and the following datasets related to 61 animals from Experiment B: animal signalment and pathogen burden; raw RNA-Seq reads from skin, liver and spleen tissues; de novo assembled transcriptomes for each tissue type; raw gene expression data; annotation data for each gene; and raw metabolite expression data from skin and liver tissues. These data provide an extensive baseline for future analyses.请注意，本数据集相关内容亦见于另一篇已发表文献，请参阅http://dx.doi.org/10.1111/mec.14493。真菌性皮肤病壶菌病（chytridiomycosis）已在全球范围内造成数百种两栖动物种群的灾难性衰退与灭绝，但宿主演化出抗性的潜力及其潜在病理生理机制仍未被充分阐明。我们开展了两项并行受控感染实验，将来自多个种群（其中一个种群在演化上未接触过壶菌病）的406只未接触过病原体的人工繁育高山雨蛙（Litoria verreauxii alpina）暴露于致病菌蛙壶菌（Batrachochytrium dendrobatidis）。本研究旨在（A）解析不同种群与产卵群间的存活结局与临床病原体负荷，（B）探究宿主个体对壶菌病的组织应答。本数据集包含与上述暴露实验相关的多组关联数据：其中实验A涵盖355只个体的动物信息（signalment）、存活情况及病原体负荷数据；实验B涉及61只个体的相关数据，包括动物信息与病原体负荷、皮肤、肝脏与脾脏组织的原始RNA-Seq读段、各组织类型的从头组装转录组、原始基因表达数据、各基因注释数据，以及皮肤与肝脏组织的原始代谢物表达数据。本数据集为后续相关分析提供了丰富的基础资源。