Quantitative Analysis of Lysine Acetylation in A549 Cell Line

C-C Motif Chemokine 18 (CCL18) belongs to the chemokine CC family and is predominantly secreted by M2-tumor associated macrophage (TAMs). It has been reported to be associated with various diseases and malignancies. Previous study showed that CCL18 promotes metastasis by activating downstream kinases. However, it remains unknown whether CCL18 regulates post-translational modifications, other than phosphorylation, during tumorigenesis. Here, we demonstrate that CCL18 is up-regulated in non-small cell lung cancer (NSCLC) and is involved in regulating the lysine acetylome in A549 cells. Using the combination of SILAC labeling and high-efficiency acetylation enrichment methods