Transcriptome analysis of the iron regulatory transcription factors in Cryptococcus neoformans in response to iron availability. Transcriptome analysis of the iron regulatory transcription factors in Cryptococcus neoformans in response to iron availability

Purpose: Understanding the iron-responsive regulatory networks in Cryptococcus neoformans. Methods: The transcriptome profiles of the wild-type, cir1 mutant and hapX mutant were generated by RNA-seq using Illumina Hiseq, in triplicate. The transcriptome profiles of each mutant was compared with that of the wild-type strain. Results: The iron-responsive transcription factors, Cir1 and HapX, are major regulators for iron acquisition and metabolism in C. neoformans. Overall design: Transcriptome of each mutant vs. Transcriptome of the wild-type