Kinesin-1 and dynein at the nuclear envelope mediate the bidirectional migrations of nuclei

DIC images from a representative time-lapse sequence of klc-2(km11) nuclear migration in hyp7 precursors. Cellular boundaries appear as depressions in the hypodermis and nuclei are exclusions of cytoplasmic granules. Embryos were dissected from gravid hermaphrodites and mounted on a 2% agar pad in M9 solution. Time-lapse microscopy of nuclear migration was observed using DIC optics and a 63x PLAN APO 1.40 NA objective on a Leica DM 6000 compound microscope. Images were acquired using a Leica DC350 FX camera and the LAS AF software (Leica) at a rate of 15 sec/frame.