Table S1. List of the up-regulated proteins found in the secretome of IL-1β primed MSC.

In the present study, we found that, IL-1β-primed gingival MSCs (IL-MSCs) promoted cell migration, dermal-epidermal junction formation and inflammation reduction in vitro, as well as improved epidermal substitute engraftment in vivo. IL-MSCs had different secretory profiles from naive gingival MSCs (NV-MSCs), characterized by an overexpression of TGF-β and MMP pathway agonists. Eventually, MMP-1, MMP-9 and TGF-β1 appeared to be critically involved in IL-MSC mechanisms of action.

本研究发现，经白介素1β（IL-1β）预处理的牙龈间充质干细胞（gingival Mesenchymal Stem Cells，MSCs，简称IL-MSCs）可在体外促进细胞迁移、真皮-表皮连接形成及炎症缓解，并在体内提升表皮替代移植物的植入成活率。IL-MSCs与未经过预处理的牙龈间充质干细胞（NV-MSCs）的分泌谱存在显著差异，其特征为转化生长因子β（TGF-β）及基质金属蛋白酶（MMP）通路激动剂的过表达。最终研究表明，基质金属蛋白酶1（MMP-1）、基质金属蛋白酶9（MMP-9）及转化生长因子β1（TGF-β1）在IL-MSCs的作用机制中发挥关键作用。