SvevoZavitan_introgression_line_genotyping

The goal of this study was to investigate if the introduction of small wild emmer introgressions can expand the range of phenotypic response to water stress in an elite wheat cultivar background. To accomplish this, we developed a set of introgression lines (ILs) using elite durum wheat cultivar, Svevo as the backcross parent, and Zavitan as the source of wild emmer introgressions (Avni et al., 2014). Zavitan is well-adapted to the semi-arid environment and has a sequenced and annotated genome thus making it more accessible for downstream genetic analysis compared to other wild emmer accessions (Avni et al., 2017). The subset of ILs selected for this study by eliminating the wild alleles for the Reduced height (Rht)-B1b gene and non-brittle spike (TtBtr1) genes. This set included 47 wild emmer ILs were genotyped using the 90K SNP chip, and poses 1.3-14.2% of the Zavitan genes per IL.