Composition and quantification of Polycyclic Aromatic Hydrocarbons in deep-pelagic fauna. Cruises DP02, DP03, and DP04 from August 2015 - August 2016

Samples were collected in the northern Gulf of Mexico on cruises DP02, DP03 and DP04 from August 2015 - August 2016. Polycyclic Aromatic Hydrocarbons (PAH) data were integrated with those from population dynamics and stable isotope analysis to provide evidence for effects of oil exposure to deep pelagic communities. The target fish species include Argyropelecus spp., Ceratoscopelus warmingii, Chauliodus sloani, Diaphus spp., Lampanyctus alatus, Lepidophanes guentheri, and Sigmops elongatus. Analysis of PAHs was conducted on different fish tissues (muscle, guts, gills, and eggs) following modified EPA method 8270D and QA/QC strict protocols. Detection and quantification of PAH homologues was performed by using a gas chromatograph/mass spectrometric detector (GC/MS/MS). Percent lipid content was measured by calculating the total lipid fraction in GCMS chromatograms.本数据集的样本采集于2015年8月至2016年8月期间的DP02、DP03及DP04航次，采样区域为墨西哥湾北部海域。多环芳烃（Polycyclic Aromatic Hydrocarbons, PAH）数据与种群动态、稳定同位素分析数据相整合，旨在为石油暴露对深海远洋群落的影响提供实证支撑。本次研究的目标鱼类物种包括银斧鱼属（Argyropelecus spp.）、沃氏角肩灯鱼（Ceratoscopelus warmingii）、斯隆蝰鱼（Chauliodus sloani）、灯笼鱼属（Diaphus spp.）、翼光鱼（Lampanyctus alatus）、贡氏鳞光鱼（Lepidophanes guentheri）以及长形西格灯鱼（Sigmops elongatus）。多环芳烃的分析针对不同鱼类组织（肌肉、肠道、鳃及鱼卵）开展，实验流程参照改良后的美国环境保护署（Environmental Protection Agency, EPA）方法8270D，并严格遵循质量保证与质量控制（QA/QC）规范。多环芳烃同系物的检测与定量采用气相色谱-串联质谱检测器（Gas Chromatograph/Mass Spectrometric Detector, GC/MS/MS）完成。脂质百分含量通过计算气相色谱-质谱（GCMS）色谱图中的总脂质组分测得。