Expression data from 5-week-old mouse prostate

Heparan sulfate (HS) is a linear, sulfated polysaccharide, and expresses abundantly in prostate and PCa tissues. Intriguingly, the HS content and sulfation modifications appear to increase when the prostate becomes malignance, suggesting that HS may critically modulate PCa pathogenesis. We specifically ablated Ext1, the enzyme that initiates HS biosynthesis, in mouse prostate at late development stage. And used microarray to detail the gene expressions affected by Ext1 ablation and identified distinct classes of up-regulated genes during this process. Prostate tissues were removed from euthanized mice at 5 weeks old for RNA extraction and hybridization on Affymetrix microarrays. We sought to compare gene expression during tumorigenesis among the wildtype, prostate specific knockout of Pten and double knockout of Pten and Ext1 in prostate.硫酸乙酰肝素（Heparan sulfate, HS）是一类线性硫酸化多糖，在前列腺组织与前列腺癌（Prostate Cancer, PCa）组织中广泛高表达。值得关注的是，当前列腺发生恶性转化时，HS的含量与硫酸化修饰水平均显著升高，这提示HS可能在前列腺癌的发病机制中发挥关键调控功能。 我们在小鼠前列腺发育的晚期阶段，特异性敲除了启动HS生物合成的关键酶编码基因Ext1，随后通过微阵列芯片技术详细解析了Ext1敲除所影响的基因表达谱，并在此过程中鉴定出多类显著上调的基因。 本实验于5周龄小鼠安乐处死后获取其前列腺组织，用于RNA提取并在Affymetrix微阵列芯片上完成杂交。我们旨在比较野生型、前列腺特异性Pten敲除型，以及前列腺同时敲除Pten与Ext1的小鼠在肿瘤发生进程中的基因表达差异。