Mating types and Reproductive success

Reproductive success of 239 crosses (478 matings). Columns represent the 16 mat A strains, and rows represent the 15 mat a strains, with isolates numbers along the row and column headings of the matrix. Numbers within matrix cells indicate the reproductive success ratings (see below) of the two reciprocal matings of the cross between the corresponding isolates (mat a isolate as the perithecial parent/mat A isolate as the perithecial parent). The matrix cells have been shaded in proportion to the reproductive success of the best mating (see Figure 5B in the corresponding publication). Additional row and column headings indicate the lineage designation (see Figure 1). Isolates P581 and TX8127 are tester strains of N. discreta sensu stricto. Categories of reproductive success, corresponding to different stages in reproductive development, from the development of perithecia, to the formation of black ascospores were scored as follows: 0 & 1 if sterile, no perithecia produced; & barren perithecia, no ostiole developed; 2 if perithecia developed ostioles, but no spores; 3 & 4 if <1% black ascospores ; & 1-15% black ascospores; 5 if 15-50% black ascospores; 6 if > 50% black ascospores.本数据集涵盖239组杂交组合（共478次交配）的繁殖成功率数据。矩阵的列代表16株交配型A（mat A）菌株，行代表15株交配型a（mat a）菌株，矩阵的行列表头标注了对应分离株的编号。矩阵单元格内的数值代表对应分离株之间两组正反交组合的繁殖成功率等级（详见下文），其中两组正反交分别以交配型a分离株作为子囊壳（perithecium）亲本、交配型A分离株作为子囊壳亲本。矩阵单元格的着色深浅与该杂交组合中最优交配的繁殖成功率成正比（详见对应发表文献中的图5B）。额外增设的行列表头用于标注菌株的谱系名称（详见图1）。分离株P581与TX8127为狭义离散脉孢霉（N. discreta sensu stricto）的标准测试菌株。繁殖成功率的等级划分对应生殖发育的不同阶段，覆盖从子囊壳形成到黑色子囊孢子生成的全过程，评分标准如下：0级与1级均为不育类型，其中0级指未产生任何子囊壳，1级指形成不育子囊壳但未发育出子囊孔（ostiole）；2级为子囊壳可形成子囊孔，但未产生孢子；3级与4级分别对应产生<1%的黑色子囊孢子、1%~15%的黑色子囊孢子；5级对应产生15%~50%的黑色子囊孢子；6级对应产生>50%的黑色子囊孢子。