Brycon_orbignyanus_Assembly_1_A1_k55.fsa

Supplementary Data S3: Brycon orbignyanus (Characiformes: Bryconidae) draft partial genome assembly (~10X) in fasta format (.fsa). Assembled from Illumina HiSeq 2000 short reads (retrievable from NCBI-SRA SRX3350440), using SOAPdenovo 2 (k-mer=55) with the intention to screen for microsatellite loci. This assembly A1 was the base for the alignments performed with SOAPaligner retrivable from NCBI-SRA SRX3427716).
The final size of the genomic assembly A1 is 1,113,754,917 bp (including unknown base calls and gaps, N) and 1,039,212,289 bp (not counting Ns – Δ=74,542,628). A total of 1,273,306 contigs or scaffolds were obtained, the shortest being 100 bp and the longest 172,138 bp (average=874). Only 55 scaffolds were longer than 100 kbp; 1.97% longer than 10 kbp; 11% longer than 1 kbps and 18.09% of 500 bp or more. The A1 assembly had the CG content of 41.18% and N50=8,463.
These results are part of the article "A broad genomic panel of microsatellite loci from Brycon orbignyanus (Characiformes: Bryconidae), an endangered migratory Neotropical fish". Scientific Reports, 8: 8511, 2918