Supplemental Materials for "Future Sequon Finder - A novel approach for predicting future N-linked glycosylation sequons on viral surface proteins"

Java Program PackagesfutureSequonFinder— This program as the main program used to predict the emergence of sequons, and is thoroughly described in the manuscript.ifvDataCleaner - This is the program used to filter the HA nucleotide sequence datasets that were used for site-specific mutation frequency analysis.MultipleSequonFinder - This program was used to determine when specific glycosylation sequons first emerged in the H1N1 swine dataset.Phylogenetic TreesThis folder contains the newick files encoding phylogenetic trees for the human H1N1 and H3N2 analysis strains referenced in the manuscript. The H1N1 swine tree contains all swine analysis strains and an additional ≤25 strains per year for context.Supplementary PDB FilesThis folder contains all PDB files generated by Alpha Fold for the related strains immediately preceding glycosylation events that Future Sequon Finder would analyze. The human H1N1, H3N2, and swine H1N1 structures are included here.Influenza_Analysis_SequencesThis Excel spreadsheet contains details for all influenza analysis strains (those strains that either directly precede a sequon addition event or have obtained a new sequon). Their names, nucleotide sequences, database, phylogenetic group, analysis group, and host species are all included.