Col-HA defect images

Data associated with article: Effects of cell-attachment and extracellular matrix on bone formation in vivo in collagen-hydroxyapatite scaffolds Max M. Villa, Liping Wang, David W. Rowe, and Mei Wei Department of Materials Science &amp; Engineering, 97 North Eagleville Road, University of Connecticut, Storrs, CT 06269 Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 This dataset contains histological sections of critical size calvarial defects filled with collagen-hydroxyapatite scaffolds and culture expanded bone marrow osteoprogenitors after three weeks in vivo. Naming scheme: Group_AnimalEarNotch-Slide#_replicate#Channel#+Channel#+Channel#.jpg example:<br>G1-NB-1_s1c2.jpg Explanation:<br>Group<br>G1 - samples incubated in vitro overnight before implantation<br>G2 - samples incubated in vitro overnight before implantation +BME gel used as a seeding suspension<br>G3 -samples implanted immediately after seeding<br>G4 -samples implanted immediately after seeding +BME gel used as a seeding suspension AnimalEarNotch<br>G1&amp;G2<br>NB - ear notch both<br>NL - ear notch left<br>NR - ear notch right<br>HR - hole right<br>HL - hole left<br>HB - hole both<br>G3&amp;G4<br>NB - ear notch both<br>NL - ear notch left<br>NR - ear notch right<br>HR - hole right<br>HL - hole left<br>HB - hole both<br>Slide Number<br>Three tissue sections were placed on a slide. Slide number can be a value 1, 2 or 3.<br>Replicate number<br>One the replicate serial sections of the same calvarium, can be a value s1, s2 or s3.<br>Channel number<br>Corresponds to 1 of the 3 channels acquired:<br>Channel1-c1- DIC, grayscale<br>Channel2-c2- calcein mineralization label, green<br>Channel3-c3- Tdtomato, donor cells, red