Additional file 2: of Identification of transcription factor genes involved in anthocyanin biosynthesis in carrot (Daucus carota L.) using RNA-Seq

Sequence and primer properties used to analyses 11 candidate genes and GAPDH (Additional file 2.1), PCR efficiencies calculated for primer pairs using 4 fold (based on mean Ct values observed for triplicates) dilution series of cDNA mix from all 30 samples (Pfaffl 2001; Additional file 2.2), relative expression ratio of 11 candidate genes in purple vs non-purple calli/tissue (Average of biological samples) calculated with following formula (Pfaffl 2001; Additional file 2.3); trimmed mean of M-values (TMM) of 11 candidate genes in purple and non-purple calli/tissue (Additional file 2.4). (XLSX 58 kb)