De novo assembly and transcriptome analysis of Litopenaeus vannamei gill response to different salinity cultivated conditions

We provided a step forward in examining the transcriptomic response of L. vannamei to different salinity conditions following the application of the next-generation sequencing techniques and was first to explain osmoregulation of L. vannamei at a transcriptome-wide scale based on three practical cultivated models with different salinities. The assembled sequence data comprising 349,012 unique transcripts were obtained, and the established transcriptional database provided a comprehensive sequence source for this non-model organism. Gill mRNA profiles of L. vannamei cultured in seawater, brackish water and fresh water, respectively, were generated by deep sequencing, in triplicate, using Illumina GAIIx.