Reduced representation bisulfite sequencing of liver DNA of 4-week old Bhmt-null mice (N=8) and wild type controls (N=8)

We measured the effect of methylation potential decrease, characteristic to the betaine homocysteine methyl-transferase (Bhmt) null mice, on liver DNA methylation patterns at 4 weeks. We used reduced representation bisulfite sequencing (RRBS) to measure DNA methylation differences in the livers of Bhmt-null and wild type mice (n= 8/group). We filtered sequencing data for CpGs with at least 10x coverage.