Supporting Information S1 - Gender Differences in Transcriptional Signature of Developing Rat Testes and Ovaries following Embryonic Exposure to 2,3,7,8-TCDD

Reproductive parameters of the female progeny exposed in utero to TCDD. (a) F1 female progeny weight from 5 to14 postnatal days. Values (g) are mean ± SEM of () number of pups. TCDD-200 ng females were significantly lighter than control females at 4, 7 and 10 postnatal days (* p(b) F1 female fertility assessment. Control females (7) and TCDD-200 ng females (5) were mated continuously with males from 2 to 7 months of age. A total of 5 litters was obtained for each female. Newborn pups were sacrificed after 2 days to check viability. For each litter, the mean number of days between beginning of mating and parturition is indicated for the 7 control and 5 TCDD-treated females. We also recorded the mean number of pups of each sex for the 7 control and 5 TCDD-treated females. (c) Expression levels of key genes involved in endocrine function of the ovary. No significant differences were observed between control and TCDD-200 ng treated females assessed through a transcriptomic analysis on 14 dpn ovaries. Values are the mean of the 3 samples analysed by microarray. (d) Real-time RT-PCR measurement of Cyp19a1 and Star genes in ovaries during prepubertal period. Values were normalized using Hprt and are mean ± SEM of () number of ovaries. No significant differences were observed between control and TCDD- 200 ng treated females assessed from 3 to 25 postnatal days (dpn). (DOC)