Mouse SINE B2 elements function as IFN-inducible enhancer elements [CUT&Tag]

B2_Mm2 acts as a STAT1 binding site and interferon inducible enhancer of the nearbby interferon-stimulated gene Dicer1 during an innate immune response (IFNG stimulated). J774 knockouts deficient of B2_Mm2 were also found to have downregulated expression of Serpina3f and Serpina3g, indicating that B2_Mm2 acts as an enhancer on these nearby genes likely due to its capability as a CTCF-binding site. Overall design: Chromatin profiling (CUTnTag) of wildtype mouse cell lines (J774) that were stimulated with 100 ng/mL interferon gamma (IFNG) for 4h. CUTnTag is also provided from clonal B2 knockout J774 lines that were modified using CRISPR Cas9 and a pair of gRNAs targetting the B2_Mma2 element in the first intron of Dicer1 on chr12.