Growth Optimized Aminoacyl-tRNA Synthetase Levels Prevent Maximal tRNA Charging. Parker et al

tRNA charging Northern blot to demonstrate increased charging of tRNA with cognate aaRS overproduction. One .tif file of multiple blots imaged together. Intensity was changed in figure to aid viewing. In order from left to right: Probed for argACG tRNA: Lane 1 - Deacylated control sample Lane 2 - WT 168 sample Lane 3 - ArgS overproduction sample Lane 4 - ArgS overproduction sample Replicate Lane 5 - SerS overproduction sample Probed for serGCU tRNA: Lane 1 - Deacylated control sample Lane 2 - WT 168 sample Lane 3 - SerS overproduction sample Lane 4 - IleS overproduction sample Probed for ileCAU tRNA: Lane 1 - Deacylated control sample Lane 2 - WT 168 sample Lane 3 - IleS overproduction sample Lane 4 - LeuS overproduction sample Probed for leuUAA tRNA: Lane 1 - Deacylated control sample Lane 2 - WT 168 sample Lane 3 - LeuS overproduction sample Lane 4 - IleS overproduction sample本数据集为验证同源氨酰-tRNA合成酶（aminoacyl-tRNA synthetase, aaRS）过量表达可提升转运RNA（transfer RNA, tRNA）氨酰化水平的Northern印迹杂交（Northern blot）实验数据。实验包含一张整合了多张印迹结果的.tif格式图像文件，为便于观察已对图像亮度进行调整。按从左至右的顺序，各组探针检测详情如下： 1. 针对argACG tRNA的探针检测： 泳道1：去酰化对照样品 泳道2：野生型168菌株样品 泳道3：ArgS过量表达样品 泳道4：ArgS过量表达重复样品 泳道5：SerS过量表达样品 2. 针对serGCU tRNA的探针检测： 泳道1：去酰化对照样品 泳道2：野生型168菌株样品 泳道3：SerS过量表达样品 泳道4：IleS过量表达样品 3. 针对ileCAU tRNA的探针检测： 泳道1：去酰化对照样品 泳道2：野生型168菌株样品 泳道3：IleS过量表达样品 泳道4：LeuS过量表达样品 4. 针对leuUAA tRNA的探针检测： 泳道1：去酰化对照样品 泳道2：野生型168菌株样品 泳道3：LeuS过量表达样品 泳道4：IleS过量表达样品