Code from: First insights into female sperm storage duration in tardigrades

Female sperm storage is ubiquitous in the animal kingdom and it has been shown to be linked to several evolutionary processes, from postcopulatory sexual selection to dispersal. Here we report, for the first time, long-term sperm storage in females of the Macrobiotus polonicus tardigrade. Females, isolated after a short contact with a male, were able to use the stored sperm for up to 5 weeks (mean of 2 weeks), which translates to a considerable proportion of female post-mating longevity under controlled laboratory conditions (60% on average). Our study provides first insights into the duration of sperm storage, an underexplored feature of the reproductive biology of tardigrades. Additionally, we discuss important considerations for reproductive studies on these non-model animals., , , # Code from: First insights into female sperm storage duration in tardigrades

[https://doi.org/10.5061/dryad.fn2z34txb](https://doi.org/10.5061/dryad.fn2z34txb)

#### Data and file structure

There are two data files in comma-separated format: data1_ovipositions.csv: This file records individual egg-laying events for females over time. Each row is one oviposition check, with dates, number of eggs laid, timing since first/previous oviposition, batch info, and interval censoring variables used to estimate when oviposition actually occurred. and data2_matingtrials.csv: This file summarizes female life-history and mating outcomes. Each row is one female, with birth and mating dates, age at mating, whether she laid eggs, total eggs produced, first/last oviposition intervals, and death timing (with censoring).

These are downloadable but incorporated as part of the script file (see below).

#### Code

The complete R script and output of the statistical analyses are all incorporated into the...,