Martin Wuehr, Timothy J. Mitchison (2010) CIL:9686, Xenopus laevis. CIL. Dataset

Frog (Xenopus laevis) egg arrested in metaphase of mitosis. Note the tiny metaphase spindle on the top left of the egg.

Eggs were fixed in 90% MeOH; 10% (H20 500mM EGTA) and stained for DNA with YoPro1 (green) and tubulin (red) with antibody B-5-1-2 directly labeled with Alexa 647. A single z slice was collected with a Zeiss LSM 710 using a 10 X 0.3NA objective. Lasers were 488 (DNA stain) and 633 (Tubulin stain).

非洲爪蟾（Xenopus laevis）卵母细胞处于有丝分裂的中期。请注意卵母细胞左上方的微小中期纺锤体。卵母细胞以90%甲醇进行固定，并使用10%的（H2O 500mM EGTA）进行染色，以YoPro1（绿色）染色DNA，以及用抗体B-5-1-2直接标记的Alexa 647染色微管（红色）。使用蔡司 LSM 710显微镜以10 X 0.3NA物镜收集单个z切片。激光波长分别为488nm（DNA染色）和633nm（微管染色）。