Male mouse recombination maps for each autosome identified by chromosome painting

Linkage maps constructed from genetic analysis of gene order and crossover frequency provide few clues to the basis of the genomewide distribution of meiotic recombination, such as chromosome structure, that influences meiotic recombination. To bridge this gap, we have generated the first cytological recombination map that identifies individual autosomes in the male mouse. We prepared meiotic chromosome (synaptonemal complex [SC]) spreads from 110 mouse spermatocytes, identified each autosome by multicolor fluorescence in situ hybridization of chromosome- specific DNA libraries, and mapped 12,000 sites of recombination along individual autosomes, using immunolocalization of MLH1, a mismatch repair protein that marks crossover sites. We show that SC length is strongly correlated with crossover frequency and distribution. Although the length of most SCs corresponds to that predicted from their mitotic chromosome length rank, several SCs are longer or shorter than expected, with correspond..., SC Spreads and Immunostaining
Three juvenile (20–21 d old) C57BL/6J mice (the same line analyzed by the Mouse Genome Sequencing Project) were used to prepare and immunolabel the SC spreads, as described elsewhere (Anderson et al. 1999). Complete sets of SCs in which the SCs were well separated but not obviously stretched or broken and that had ≥ 19 MLH1 foci were selected for analysis. Three fluorescent images (4, 6-diamino-2-phyenylindole [DAPI], SCP3, and MLH1) were captured for each SC set.
mFISH
After image acquisition of the immunofluorescence signals, the spermatocyte preparations were subjected to two or three rounds of denaturation and FISH. To identify each autosome, chromosome-specific painting probes (Rabbitts et al. 1995) were combinatorially labeled with fluorescein isothiocyanate (FITC)–2-deoxyuridine 5-tri phosphate (dUTP), Cy5-dUTP (both from Amersham), or 6-carboxytetramethylrhodamine (TAMRA)-dUTP (Applied Biosystems) and were combined to form two different probe pools ..., , # Male mouse recombination maps for each autosome identified by chromosome painting

## Description of the data and file structure

The data are presented in an Excel spreadsheet with 22 sheets.  Sheet 1 (karyotype-absolute positi calc) defines the average length of each mouse SC, after identification using chromosome-specific DNA probes.  Sheet 2 (Notes) contains definitions of the headings used for Sheet 3 (raw data sorted by SC) and Sheets 4 through 22 (\"SC1 abs\" through \"SC19 abs\"). Sheet 2 also contains explanations for how the karyotype was derived, and two references in which this data was used for publication are also presented.  Sheet 3 contains the positions of all MLH1 foci observed on all of the SCs with each MLH1 focus position expressed as a fraction of SC length from the centromere.  Sheets 4 – 22 (labeled as \"SC1 abs\", \"SC2 abs\", \"SC3 abs\", \"SC4 abs\", SC5 abs, \"SC6 abs\", \"SC7 abs\", \"SC8 abs\", \"SC9 abs\", \"SC10 abs\", \"SC11 abs\", \"SC12 abs\", \"SC13 abs\", \"SC14 abs\", \"SC15 ...