Plant-produced viral nanoparticles decorated with nanobodies against HER2 improve retention and recruitment of immune cells in solid tumors

Potato virus X (PVX), a filamentous, positive-sense RNA plant virus, has been engineered into a molecular tool for diverse biotechnological applications, including cancer cell targeting. Here, we present the production and functional characterization of genetically-encoded PVX-derived nanoparticles decorated with nanobodies targeting two common receptors in human cancer cells, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2). We first generated a series of PVX-derived nanoparticles displaying distinct nanobodies against EGFR and HER2 in Nicotiana benthamiana plants. Self-assembly and structural integrity of the recombinant nanoparticles were confirmed by immunogold electron microscopy. We next characterized in vitro the cancer-cell binding capacity of the different recombinant viral nanoparticles (VNPs) by flow cytometry and confocal microscopy. Best performing VNPs were further assayed in vivo using tumor-bearing mice. Results showed that nanobody deco..., , # Data from: Plant-produced viral nanoparticles decorated with nanobodies against HER2 improve retention and recruitment of immune cells in solid tumors

Dataset DOI: [10.5061/dryad.x69p8czzn](https://doi.org/10.5061/dryad.x69p8czzn)

This contains the data used for experimental analysis for the article: Plant-produced viral nanoparticles decorated with nanobodies against HER2 improve retention and recruitment of immune cells in solid tumors (Authors: Enrique Lozano-Sanchez#, Miguel A. Moreno-Gonzalez#, Fernando Merwaiss, Nicole F. Steinmetz, and José-Antonio Daròs).

These data support experimental efforts to engineer Potato virus X (PVX)–derived nanoparticles functionalized with nanobodies targeting EGFR and HER2 and to evaluate their structural integrity, cellular binding behavior, and biological performance. Data were generated from plant-based nanoparticle production, electron microscopy, in vitro cell-based assays, and in vivo studies in mouse tumor models.

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