PrimaryScreen.tsv

The raw screening results are presented in a tab-separated values file. Each row represents an experiment from a single well. Plate. A unique ID for a 96-well plate on a given date. Well. A number assigned to each well of the 96-well plate. The wells are sequentially numbered with the upper-leftmost well assigned as 1 and the lower-leftmost well assigned as 85 (see Figure 3) Concentration. The concentration of the odorant applied in uM. A “9999” indicates no odor was applied (DMSO was diluted 1:10000 in CD293). Luc. The number of photons counted by the plate reader when the well was treated with the luciferase substrate. This is the cAMP reporter, and therefore correlates with receptor responses to odorants. RL. The number of photons counted by the plate reader when the well was treated with the Renilla luciferase substrate. This is the constitutively active reporter, which serves as a control for cell death and transfection efficiency. OR. A unique ID for each olfactory receptor clone Odor. A unique ID for the odorant applied to the well Date. The date the experiment was run in MM/DD/YY format.