Genome-wide maps of chromatin state in human primary CAR-T cells [scRNA-seq]

We report the single-cell transcriptomic profile of human primary T cells specifc for NY-ESO-1. CRISPR gene editing was used to delete MED12 or the "safe harbor" control gene AAVS1. T cells were transduced with a lentiviral vector encoding alpha and beta chains of the NY-ESO-1 TCR, then cultured in vitro for 10 days and adoptively transferred into A375 tumor bearing mice. Tumors were harvest 6 days following adoptive transfer. Tumors were dissociated and antigen-specific T cells were isolated by FACS.