Apolipoprotein A-IV reduced metabolic inflammation in white adipose tissue by inhibiting IKK and JNK signaling in adipocytes

ApoA-IV produced by enterocytes as a major component of high-density lipoprotein plays a role in the satiation signal and also the transport of serum lipids. The present study aimed to investigate the effects of ApoA-IV on diet-induced obesity (DIO), metabolic inflammation and the underlying mechanisms. C57BL/6J ApoA-IV-/- (KO) and wildtype (WT) mice as well as KO mice stably transfected with ApoA-IV-green fluorescent protein (KO-A4-GFP) or solely GFP (KO-GFP) encoding adenoviruses were fed with standard 5% fat rodent chow (CD) or 60% high fat rodent chow (HFD) for 16 weeks. Overall design: RNA-seq profiling of ApoA4 knockdown or WT mice, fed high fat diet or chow diet. Total RNA was isolated from eWAT tissue samples taken from 21-week-old mice (n = 4 per group) . After fragmentation and conjugation with sequencing adapters, the cDNA libraries were sequenced on a BGI-seq 500 platform using the PE100 strategy