Data generated through the identification of potential novel RNA targets for box C/D snoRNAs in budding yeast

This dataset relates to a study which identified potential novel RNA targets for box C/D snoRNAs in budding yeast, using the approach of UV crosslinking and sequencing of hybrids (CLASH) with the snoRNP proteins Nop1, Nop56 and Nop58; as well as the development of a bioinformatics approach to filter snoRNA-target interactions for bona fide methylation guide interactions.<br>The dataset consists of two zipped file folders and a single spreadsheet in .xls format. The zipped folder <b>Dudnakova et al. Supp dataset 1.zip </b>contains two .eps files, and the zipped folder <b>Dudnakova et al. Supp dataset 3.zip</b> contains the subdirectory <b>qPCR data for paper</b> which consists of ten additional spreadsheets in .xls format.<br><b>Folder Supp dataset 1.zip</b> contains two image files in .eps format. The file <b>Supplementary Data 1A.eps</b> contains raw images of northern blots, showing northern analysis of pre-rRNA processing in the strains expressing mutant U3 and marked as A, B, C and D:<br>A. Hybridized with probe 04 (5’GGTTTTAATTGTCCTA) binding to the site is located in the ITS1 region 5’ to site A2.B. Hybridized with probe 03 (5’TGTTACCTCTGGGCCC) binding the site located in the ITS1 region of the pre-rRNA, 3’ to cleavage site A2.C. EtBr staining of the membrane showing 25S and 18S rRNAs.D. Northern analysis of pre-rRNA processing following transfer of the U3mutant strain to glucose medium for 36h.<br>The file <b>Supplementary Data 1B.eps</b> contains raw images of northern Blots from RNA IP experiment in U3 mut expressing cells, marked as A, B, C and D:<br>A. Northern blot showing expression of the wild type (U3 wt) and the truncated U3 (U3 mut). B. RNA IP northern blot from the strains expressing either TAP-Nop1 or TAP-Rrp9 and mutant U3.C. Northern blot showing expression of the wild type (U3 wt) and the truncated U3 (U3 mut). Lanes 1-3 and 4-6 for show biological triplicates.D. RNA IP northern blot from the strains expressing either TAP-Nop1 or TAP-Rrp9 and mutant U3.<br>The spreadsheet <b>Dudnakova et al. Supp dataset 2.xlsx</b> contains values for growth of the strains in the growth media indicated. <br>The zipped folder <b>Supplementary Dataset 3.zip </b>contains ten spreadsheets in .xls format, which show the Ct values obtained for the mRNA species indicated.<br>The .xls spreadsheets can be opened using Microsoft Excel software, and the .eps image files can be opened using graphic software such as Adobe Illustrator or alternatively Adobe Acrobat.<br><br>