CRISPR library targeting lncRNA and protein coding genes along BLaER1 transdifferentiation (Replicate 2)

CRISPR library screening experiment in BLaER-Cas9 cells that were induced to transdifferentiate into macrophages. The purpose of the experiment is to discover novel players (lncRNAs and protein-coding genes) in the B-cell to macrophage transdifferentiation process. Conclusion: There is a delayed population of cells that do not differentiate normally (this population is separated by cell sorting) in comparission to a control experiment with cells infected with a intergenic pgRNA Notes: The experiment was done in 2 biological resplicates (R1 and R2). BLaER Cas9 infected with non-targeting pgRNA negative control.