Leptospira spp. used during this study.

@WHO/FOA/OIE/ Collaborating Center for Reference and Research on Leptospirosis, Australia;*Bureau of Emerging Infection Disease, Ministry of Public Health, Thailand;#American Type Culture Collection, USA;$Royal Tropical Institute (KIT), Netherland;aDr Thareerat Kalambaheti, Mahidol University, Thailand.+ and − indicate a positive or negative result in the relevant PCR assay, respectively. (+) indicates a positive PCR result at a high Cq.