Gene expression profiles of healthy mononuclear cells and eosinophils and asthma (moderate and severe) eosinophils

We used scRNAseq to better characterize human eosinophils at resting state in healthy patients (compared to other MNCs). We then compared the healthy eosinophils to eosinophils from asthmatic patients (moderate and severe). Sample preparation was optimized to isolate and process human eosinophils, followed by analysis by scRNAseq.