RNA sequencing approaches to identify the differential expression gene in the mPFC with AAV-mediated Npas4 shRNA expression

Chronic social defeat stress induces anhedonia-like behavior deficits. We demonstrated that mPFC NPAS is requred for chronic social defeat stress-induced deficits in the sucrose preference and effort-based reward seeking behavior. We conducted the RNA-seq analysis for differential expression genes in the mouse mPFC samples of AAV-mediated Npas4 shRNA expression tissue. Overall design: Examination of mRNA expression in the mouse mPFC of AAV-mediated Npas4 shRNA and control scramble shRNA expression.