Gene expression profiles in control and Setdb1-KD PGCLCs. Mus musculus

In mice, primordial germ cells (PGCs), which are the precursors of gametes, are fate-determined from pluripotent epiblasts. The epigenetic regulation of PGC fate determination remains poorly understood. We identify histone methyltransferase Setdb1 as an epigenetic regulator of PGC fate determination by an RNA interference screen for histone modifier genes involved in PGC-like cell (PGCLC) induction in culture. To comprehensively investigate changes in gene expression following Setdb1-KD during PGCLC induction, we carried out RNA sequencing (RNA-seq) analysis to compare the transcriptome of the Setdb1-KD cells with that of the control KD cells 2 days after induction of PGCLCs