GBS data of seven Brassica cultivars from Pakistan

The genotyping-by-sequencing (GBS) data was generated on seven Brassica cultivars from Pakistan as a purpose of varietal registration. After strict quality-control, only 501 high-quality SNP markers were retained with 0% missing data. These marker sites can be used for varietal identification and diversity analysis., The DNA was extracted using CTAB method, and the sequencing library was prepared following standard guidelines of two restriction enzymes based strategy. The sequencing libraries were sequenced on T7 MGI-Seq platform. The raw sequencing reads were aligned to the Reference genome sequence of Brassica napus, and the SNPs were called using GATK variant calling pipeline. The vcf file generated is being uploaded which include 501 SNPs with 100% data availability. , , # GBS data of seven Brassica cultivars from Pakistan

[https://doi.org/10.5061/dryad.15dv41p4g](https://doi.org/10.5061/dryad.15dv41p4g)

The GBS data of seven Brassica cultivars from Pakistan including:

1\) NIA-Canola-2023

2\) Surhan

3\) Super Canola

4\) NIA Toria Gold

5\) Rainbow

6\) Super Raya

7\) Toria Selection-A.

## Description of the data and file structure

The data file consists of 501 SNPs in vcf format with SNP positions from reference genome of *Brassica napus* PRJEB5043.

## Sharing/Access information

The data is only accessible from DryAd.

## Code/Software

BWA-MEM

Samtools

GATK

GATK Haplotype Caller