supplementary data

The supplementary material provides comprehensive raw data for the mycelial growth inhibition of 30 isolates under two different fungicide treatments.​<b>Table S1:</b> Records the radial growth (mm) of isolates when treated with the multisite protectant <b>Tridex Super 75 WG</b> at concentrations of 25, 50, 75, and 100 ppm. It demonstrates a uniform, steep decline in growth across all strains.​<b>Table S2:</b> Details the response to <b>Difenoconazole</b>, highlighting the distinct subgroup of resistant isolates (e.g., TUAa51 and TUAa69) that maintained significant growth even at high concentrations.​Statistical and Phylogenetic VisualizationsAdvanced statistical modeling and validation plots are included to illustrate the differences in fungicide performance.​<b>Figures S1–S4:</b> Contain mixed-effects model plots, correlation matrices between chemical treatments, and <b>Tukey’s HSD</b> post-hoc comparison charts. <b>Cohen’s d</b> effect size plots further quantify the superior efficacy of Tridex over Difenoconazole at specific dose levels.​<b>Figure S6:</b> Presents a multiple sequence alignment of the <i>CYP51</i> gene, marking specific non-synonymous mutations (e.g., Met185Leu, Asn462Lys) that differentiate sensitive wild-type strains from resistant isolates.​Structural Bioinformatics ValidationThe document includes high-resolution validation data for the homology models used in the docking simulations.​<b>Table S3:</b> Lists the homology modeling parameters, including high <b>GMQE scores</b> (0.93) and sequence identity, which confirm the reliability of the models.​<b>Figure S5:</b> Features a <b>Ramachandran plot</b> generated by <b>PROCHECK</b>, confirming that the vast majority of amino acid residues in the refined <i>CYP51</i> models fall within the most favored and additionally allowed regions, ensuring stereochemical accuracy for subsequent docking analysis.​