k-mer matrix Aegilops tauschii diversity panel (Open wild wheat consortium phase II) Part 2/3

Wild wheat relatives of bread wheat represent genetic diversity that can be used for wheat crop improvement. We generated a k-mer presence/absence matrix of over 920 accessions of the wild wheat Aegilops tauschii, the donor of the bread wheat D genome. This dataset was generated under the aegis of Phase II of the Open Wild Wheat Consortium (www.openwildwheat.org)., The full methods are available in the related publication., , # Population genomics of the wild wheat Aegilops tauschii (Open Wild Wheat Consortium) - k-mer matrix PART 2/3- [https://doi.org/10.5061/dryad.wpzgmsbvm](https://doi.org/10.5061/dryad.wpzgmsbvm) ## **Description of the data and file structure** *k*-mer presence/absence matrix for the *Aegilops tauschii* diversity panel of over 920 accessions, including genetically redundant accessions. Resequencing of the *Ae. tauschii* accessions: We generated short-read whole genome sequencing data for 350 *Ae. tauschii* accessions.  PCR-free paired-end libraries were constructed and sequenced on an Illumina Novaseq 6000 instrument, yielding a median 8.3-fold coverage per sample (ranging from 5.87 to 16.86-fold). *k*-mer matrix generation. We developed and implemented an optimised *k*-mer matrix workflow to generate a presence/absence *k*-mer matrix for large diversity panels ([https://github.com/githubcbrc/KGWASMatrix](https://github.com/githubcbrc/KGWASMatrix)). We counted *k*-mers (*k* = 51) i...普通小麦的野生近缘种蕴含可用于小麦遗传改良的遗传多样性。本研究构建了覆盖920余份节节麦（*Aegilops tauschii*，普通小麦D基因组供体）材料的k-mer存在/缺失矩阵。本数据集由开放野生小麦联盟（Open Wild Wheat Consortium）第二阶段项目资助（www.openwildwheat.org），完整实验方法参见相关发表论文。 # 野生小麦节节麦（*Aegilops tauschii*）群体基因组学（开放野生小麦联盟）——k-mer矩阵 第2/3部分 https://doi.org/10.5061/dryad.wpzgmsbvm ## 数据与文件结构说明 本数据集包含覆盖920余份节节麦（含遗传冗余材料）多样性群体的k-mer存在/缺失矩阵。 节节麦材料重测序：本研究为350份节节麦材料生成了短读长全基因组测序数据。采用无PCR扩增的双端文库构建方案，在Illumina NovaSeq 6000测序平台完成测序，样本平均测序深度为8.3倍（区间为5.87~16.86倍）。 k-mer矩阵构建：本研究开发并优化了一套面向大型多样性群体的k-mer存在/缺失矩阵生成流程（https://github.com/githubcbrc/KGWASMatrix），对k值为51的k-mer进行计数……