Widespread dynamic DNA demethylation in response to heat stress in Arabidopsis

We analyzed the dynamics of changes in the level of DNA methylation in Arabidopsis thaliana under the influence of heat stress. For this purpose whole-genome sequencing of sodium bisulfite treated DNA was performed. The analysis was carried out at seven time points taking into account control conditions, heat stress and return to control conditions after stopping stress treatment. The analysis showed that under the influence of heat stress there is a global decrease in the level of DNA methylation in Arabidopsis thaliana in all three sequence contexts (CpG, CHG, CHH). Bisulfite sequencing of samples from seven time points in three replicates per time point: p0 – control, p1 – heat stress after 6h, p2 – heat stress after 12h, p3 – heat stress after 24h, p4 – recovery after 6h, p5 – recovery after 12h, p6 – recovery after 24h