Menin Enhances c-Myc-mediated Transcriptional Activity To Promote Cancer Progression

MYC is a master regulator of transcription in growing cells. Menin is an enigmatic protein that displays unique ability to either suppress or promote tumorigenesis in a context dependent manner. It''s interesting to ask is there any relationship between MYC and menin.Here, we used RNA-seq to study global transcriptomic expression of MYC or MEN1 knockdown HT1080 cells to investigate whether there are any correlations between MYC- and menin- regulated gene expression. Besides, we performed ChIP-seq assays for MYC and Menin binding sequences to address whether Menin and MYC share some common binding sites on chromatin. Overall design: Human fibrosarcoma HT1080 cells were transfected with MYC shRNAs, MEN1 shRNAs or non-targeting control shRNA followed by RNA extraction and sequenced on Illumina HiSeq 4000 (Homo sapiens). And also we performed MYC ChIP-seq and Menin ChIP-seq to address whether Menin and MYC share some common binding sites on chromatin.RNA Pol II ChIP-seq for NTC and shMEN1 HT1080 cell samples were used to study the effect of Menin on RNA Pol II-mediated elongation.