Nanocrystallites Modulate Intermolecular Interactions in Cryoprotected Protein Solutions

1. "fig_1a.csv": Small-angle scattering intensity I(Q) as a function of the momentum transfer Q measured for 10 mg/ml lysozyme in 23 mol% glycerol-water at different temperatures upon cooling down after subtracting the scattering intensity measured on the pure solvent. (Note: I(Q) in arbitrary units). 2. "fig_1_inset.csv": Radii of gyration as a function of temperature upon cooling and warming as extracted from fitting the I(q)s for the dilute sample with an elliptical form factor. 3. "fig_1b.csv": Small-angle scattering intensity I(Q) as a function of the momentum transfer Q measured for 200 mg/ml lysozyme in 23 mol% glycerol-water at different temperatures upon cooling down after subtracting the scattering intensity measured on the pure solvent. (Note: I(Q) in arbitrary units). 4. "fig_2a.csv": Wide-angle scattering intensity I(Q) as a function of the momentum transfer Q measured for 200 mg/ml lysozyme in 23 mol% glycerol-water for different temperatures upon cooling. (Note: I(Q) in arbitrary units). 5. "fig_2b.csv": Wide-angle scattering intensity I(Q) as a function of the momentum transfer Q measured for 200 mg/ml lysozyme in 23 mol% glycerol-water for different temperatures upon warming up. (Note: I(Q) in arbitrary units). 6. "fig_2c.csv": 2D scattering pattern in the vicinity of Q ≈ 16-20 nm-1 measured at T = 197 K during heating. 7. "fig_2d.csv": 2D scattering pattern in the vicinity of Q ≈ 16-20 nm-1 measured at T = 245 K during heating. 8. "fig_2e.csv": Temperature evolution of the I(Q) around the ice Ih[002] diffraction peak centered at Q ≈ 17 nm−1 upon cooling. 9. "fig_2f.csv": Temperature evolution of the I(Q) around the ice Ih[002] diffraction peak centered at Q ≈ 17 nm−1 upon warming up. 10. "fig_3a.csv": Temperature dependence of the Q-value of the SAXS I(Q) peak position for a 200 mg/ml lysozyme in glycerol-water solution during a deep temperature cycle down to 195 K. 11. "fig_3b.csv": Temperature dependence of the Q-value of the SAXS I(Q) peak position for a 200 mg/ml lysozyme in glycerol-water solution during a medium temperature cycle down to 225 K. 12. "fig_3c.csv": Temperature dependence of the Q-value of the SAXS I(Q) peak position for a 200 mg/ml lysozyme in glycerol-water solution during a shallow temperature cycle down to 245 K. 13. "fig_3d.csv": Temperature dependence of the Q-value of the WAXS I(Q) peak position for a 200 mg/ml lysozyme in glycerol-water solution during a deep temperature cycle down to 195 K. 14. "fig_3e.csv": Temperature dependence of the Q-value of the WAXS I(Q) peak position for a 200 mg/ml lysozyme in glycerol-water solution during a medium temperature cycle down to 225 K. 15. "fig_3f.csv": Temperature dependence of the Q-value of the WAXS I(Q) peak position for a 200 mg/ml lysozyme in glycerol-water solution during a shallow temperature cycle down to 245 K 16. "fig_4a.csv": The protein-protein structure factor S(Q) at different temperatures (T = 195-300 K) upon cooling obtained from the fits using the two-Yukawa model. 17. "fig_4b.csv": Temperature dependence of the attraction strength parameter K1 (in units of kbT) extracted from fitting the SAXS curves upon cooling down and warming up. 18. "fig_4c.csv": The two-Yukawa potential at different temperatures (T = 195-300 K) upon cooling down. 19. "fig_4d.csv": The pair distribution function g(r) at different temperatures (T = 195-300 K) derived from the modeled structure factors. Additionally, a Jupyter notebook "open-data.ipynb" which shows how to load and plot the data from the csv files in Python.1. "fig_1a.csv": 存储浓度为10 mg/ml的溶菌酶（lysozyme）在23 mol%甘油-水混合溶剂中，降温过程中不同温度下测得的小角散射（Small-Angle Scattering, SAXS）强度I(Q)随动量转移Q（momentum transfer Q）的变化数据，已扣除纯溶剂的散射背景强度。（注：I(Q)单位为任意单位） 2. "fig_1_inset.csv": 存储针对稀样品的I(Q)曲线拟合椭圆形形状因子（elliptical form factor）后，提取得到的回转半径（radius of gyration）随升降温过程的变化数据。 3. "fig_1b.csv": 存储浓度为200 mg/ml的溶菌酶在23 mol%甘油-水混合溶剂中，降温过程中不同温度下测得的小角散射强度I(Q)随动量转移Q的变化数据，已扣除纯溶剂的散射背景强度。（注：I(Q)单位为任意单位） 4. "fig_2a.csv": 存储浓度为200 mg/ml的溶菌酶在23 mol%甘油-水混合溶剂中，降温过程中不同温度下测得的广角散射（Wide-Angle Scattering, WAXS）强度I(Q)随动量转移Q的变化数据。（注：I(Q)单位为任意单位） 5. "fig_2b.csv": 存储浓度为200 mg/ml的溶菌酶在23 mol%甘油-水混合溶剂中，升温过程中不同温度下测得的广角散射强度I(Q)随动量转移Q的变化数据。（注：I(Q)单位为任意单位） 6. "fig_2c.csv": 存储加热过程中在T=197 K下测得的Q≈16~20 nm⁻¹附近的二维散射图样数据。 7. "fig_2d.csv": 存储加热过程中在T=245 K下测得的Q≈16~20 nm⁻¹附近的二维散射图样数据。 8. "fig_2e.csv": 存储降温过程中，位于Q≈17 nm⁻¹处的冰Ih[002]衍射峰附近的I(Q)随温度的演化数据。 9. "fig_2f.csv": 存储升温过程中，位于Q≈17 nm⁻¹处的冰Ih[002]衍射峰附近的I(Q)随温度的演化数据。 10. "fig_3a.csv": 存储浓度为200 mg/ml的溶菌酶甘油-水溶液在深温循环（最低至195 K）过程中，小角散射I(Q)峰位对应的Q值随温度的变化关系。 11. "fig_3b.csv": 存储浓度为200 mg/ml的溶菌酶甘油-水溶液在中温循环（最低至225 K）过程中，小角散射I(Q)峰位对应的Q值随温度的变化关系。 12. "fig_3c.csv": 存储浓度为200 mg/ml的溶菌酶甘油-水溶液在浅温循环（最低至245 K）过程中，小角散射I(Q)峰位对应的Q值随温度的变化关系。 13. "fig_3d.csv": 存储浓度为200 mg/ml的溶菌酶甘油-水溶液在深温循环（最低至195 K）过程中，广角散射I(Q)峰位对应的Q值随温度的变化关系。 14. "fig_3e.csv": 存储浓度为200 mg/ml的溶菌酶甘油-水溶液在中温循环（最低至225 K）过程中，广角散射I(Q)峰位对应的Q值随温度的变化关系。 15. "fig_3f.csv": 存储浓度为200 mg/ml的溶菌酶甘油-水溶液在浅温循环（最低至245 K）过程中，广角散射I(Q)峰位对应的Q值随温度的变化关系。 16. "fig_4a.csv": 存储通过双Yukawa模型（two-Yukawa model）拟合得到的，降温过程中不同温度（T=195~300 K）下的蛋白质-蛋白质结构因子S(Q)（structure factor S(Q)）数据。 17. "fig_4b.csv": 存储通过拟合小角散射曲线提取得到的，吸引强度参数K1（单位为kbT）随升降温过程的温度依赖关系数据。 18. "fig_4c.csv": 存储降温过程中不同温度（T=195~300 K）下的双Yukawa势能曲线数据。 19. "fig_4d.csv": 存储通过建模结构因子推导得到的，不同温度（T=195~300 K）下的径向分布函数（pair distribution function, g(r)）数据。 此外，附带Jupyter笔记本（Jupyter Notebook）"open-data.ipynb"，用于演示如何在Python环境中加载并绘制各CSV文件中的数据。