Human bone marrow-derived stromal cell behaviour after pre-conditioned sub-lethal irradiation

Direct bone marrow injection of cells into murine marrow cavities is used in a range of cell characterization assays and to develop disease models. While human bone marrow-derived stromal cells (hBMSC, also known as mesenchymal stem cells (MSC)) are frequently described in therapeutic applications, or disease modeling, their behavior following direct injection into murine bone marrow is poorly characterized. Herein, we characterized hBMSC engraftment and persistence within the bone marrow of NOD-scid interleukin (IL)-2γ-/- (NSG) mice with or without prior 2 Gy total-body γ-irradiation of recipient mice.

将细胞直接注射至小鼠骨髓腔的实验方法，广泛应用于各类细胞表征实验以及疾病模型构建。尽管人骨髓来源基质细胞（human bone marrow-derived stromal cells，简称hBMSC），亦称间充质干细胞（mesenchymal stem cells，简称MSC）在治疗应用或疾病建模领域已有大量相关报道，但这类细胞经直接注射进入小鼠骨髓后的行为特征尚未得到充分阐明。本研究中，我们针对经或未经预先实施2戈瑞全身γ射线照射的受体非肥胖糖尿病-重症联合免疫缺陷白细胞介素-2γ敲除（NOD-scid interleukin (IL)-2γ^-/-，简称NSG）小鼠，对其骨髓内hBMSC的植入定植与留存能力进行了系统表征。